PH0492 | Western二抗稀释液 Secondary Antibody Dilution Buffer for Western

货号 PH0492

名称 Western二抗稀释液

 规格 100ml

 存储 4℃保存，一年有效。
 
产品简介 

Western二抗稀释液(Secondary Antibody Dilution Buffer)可以用于Western时二抗(secondary antibody)的稀释。经本Western二抗稀释液稀释的二抗可以在1-2周内重复使用3-5次。

按照每个二抗稀释10毫升计算，一个包装的Western二抗稀释液可以稀释10个或10次二抗。

使用说明 

参考所用的二抗的使用说明，以及一抗的质量和样品中目的蛋白的含量，按照适当比例例如1:1000、1:500等比例稀释二抗。二抗稀释后即可直接用于Western。一次Western结束后，可以回收稀释的二抗，4℃保存，以用于下次的Western，通常在1-2周内可以重复使用3-5次。

注意事项 

本Western二抗稀释液经过滤除菌处理，使用过程中应尽量避免细菌污染。一旦启用后建议在一个月内用完，如果发现有沉淀或细菌污染请弃用。

Note:

1) Antibodies diluted using this buffer can be stored at 4 ºC for 6 months without reducing binding activity;

2) Do not use BSA or other serum containing reagents to dilute secondary antibodies since they may bind to BSA or serum therefore reducing antibody affinity;

3) Using TBS to dilute secondary antibodies often produces weaker staining. So use TBS only for the antibodies with high background staining or for alkaline phosphatase conjugated antibodies since phosphate is a inhibitor of alkaline phosphatase;

4) This buffer can also be used for diluting HRP conjugated antibodies since there is no sodium azide in buffer.