PH1489 | Tris-HCl缓冲液 (1mol/L,pH8.0,无菌) / 1M Tris-HCl, pH8.0

产品简介

Tris(三羟甲基氨基甲烷)为弱碱，分子式为C4H11NO3，相对分子量为121.14, 在25℃下pKa为8.1，Tris缓冲液的有效缓冲范围在pH7.0～9.2之间。Tris碱的水溶液pH在10.5左右，一般加入盐酸调节pH值至所需值，即可获得该pH值的缓冲液。常用作生物缓冲液，常用pH值为6.8、7.4、8.0、8.8，其pH值随温度变化很大。一般来说，温度每升高1℃，pH值下降0.03。1M Tris-HCl pH8.0是分子生物学中最常用的试剂之一，用来作为各种缓冲液的基础成分，调节酸碱度，例如配制Tris-EDTA溶液，TBE电泳缓冲液等。

Tris-HCl缓冲液(1mol/L,pH8.0,无菌)采用进口Tris、去离子水配制而成，经15psi高压灭菌处理，被广泛用作核酸和蛋白质的溶剂。

操作步骤：根据实验具体要求操作。

注意事项：

对人体有刺激性，请注意适当防护。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

有效期： 常温保存，12个月有效。

Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids.

"Tris" is a chemical compound used as a buffer. The full name is tris(hydroxymethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (hydrochloric acid) the buffer is called Tris HCl.