PH0501 | 牛血清蛋白V（Bovine Serum Albumin BSA）CAS号：9048-46-8

英文名称：Albumin from bovine serum，BSA，Cohn Fraction V

CAS号：9048-46-8
分子量： 约66 kD
级别：Biotech Grade
溶解度：水中溶解度为40 mg/ml, 溶液浅黄色至黄绿色。
Purity: ≥98.0%
性状：白色至淡黄色粉末
安全防护：可能会造成皮肤过敏，引起呼吸道刺激。

牛血清白蛋白，英文名称为Bovine Serum Albumin，也称Bovine albumin，或Cohn Fraction V，简称BSA。BSA有着极其广泛的实验室应用，在免疫实验中常用作封闭剂，包括ELISA、WB（Western Blot）。作为载体蛋白，将其交联于半抗原和其他弱抗原可以使它们在抗体生产中具有更强的免疫原性。在限制性内切酶消化反应中，BSA常常被用作一些酶的反应稳定剂，并且能防止它粘附到管壁和枪头上。BSA也常用于生物制药的生产过程或者作为营养物用于细胞和微生物培养。除此之外，还作为蛋白定量检测的标准品使用。

本级别的牛血清白蛋白（BSA, Standard Grade），可以满足大部分常规实验需求，如用作免疫封闭剂、组织细胞培养添加剂、蛋白/酶稳定剂以及蛋白定量标准品。

注意事项 

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运输与存储 常温运输，2-8℃冷藏保存。有效期 3年

本试剂仅适用于实验室科研用途！

Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments.

The nickname "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology that made use of differential solubility characteristics of plasma proteins.

Applications

BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Because BSA is a small, stable, moderately non-reactive protein, it is often used as a blocker in immunohistochemistry. During immunohistochemistry, which is the process that uses antibodies to identify antigens in cells, tissue sections are often incubated with BSA blockers to bind nonspecific binding sites. This binding of BSA to nonspecific binding sites increases the chance that the antibodies will bind only to the antigens of interest. The BSA blocker improves sensitivity by decreasing background noise as the sites are covered with the moderately non-reactive protein.  During this process, minimization of nonspecific binding of antibodies is essential in order to acquire the highest signal to noise ratio. BSA is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during the digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipette tips, and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA (see Bradford protein assay). BSA is used because of its ability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. Another use for BSA is that it can be used to temporarily isolate substances that are blocking the activity of the enzyme that is needed, thus impeding polymerase chain reaction (PCR).